sigmaplot 8 Search Results


sigmaplot 8.0  (SPSS Inc)
90
SPSS Inc sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pmc02615518-122-17-19?v=SPSS+Inc
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-06
90/100 stars
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sigmaplot ® 8 program pharmacology module  (SPSS Inc)
90
SPSS Inc sigmaplot ® 8 program pharmacology module
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot ® 8 Program Pharmacology Module, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pmc02848119-89-27-33?v=SPSS+Inc
Average 90 stars, based on 1 article reviews
sigmaplot ® 8 program pharmacology module - by Bioz Stars, 2026-06
90/100 stars
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sigmaplot 8.0  (Alfasoft GmbH)
90
Alfasoft GmbH sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by Alfasoft GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pmc02712688-152-0-2?v=Alfasoft+GmbH
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
sigmaplot 8.0  (HULINKS Inc)
90
HULINKS Inc sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by HULINKS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pmc01794218-197-6-8?v=HULINKS+Inc
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
sigmaplot 8.0  (SSI Inc)
90
SSI Inc sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by SSI Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pm17251307-63-14-16?v=SSI+Inc
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
sigmaplot 8.0 graphics  (SAS institute)
90
SAS institute sigmaplot 8.0 graphics
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0 Graphics, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/10__1111_slash_j__1365___2486__2009__02052__x-94-25-24?v=SAS+institute
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 graphics - by Bioz Stars, 2026-06
90/100 stars
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sigmaplot 8.02  (Rockware Incorporated)
90
Rockware Incorporated sigmaplot 8.02
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.02, supplied by Rockware Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot+8/pm15971207-102-24-26?v=Rockware+Incorporated
Average 90 stars, based on 1 article reviews
sigmaplot 8.02 - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.

Journal:

Article Title: RecR-mediated Modulation of RecF Dimer Specificity for Single- and Double-stranded DNA * S⃞

doi: 10.1074/jbc.M806378200

Figure Lengend Snippet: FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.

Article Snippet: The fluorescence polarization signal was plotted against the RecR concentration for each RecF concentration, then analyzed with SigmaPlot 8.0 (SPSS, Inc.) using a single-site ligand-interaction model and Equation 7 , (Eq.

Techniques: Titration, Labeling, Software, Derivative Assay

RecF-DNA interaction measured by the fluorescence polarization assay. The labeled DNA substrates (20 nm) were titrated with RecF in the presence of 2 mm nucleotide. RecR was included where indicated at final concentration of 30 μm. Normalized fluorescence polarization signal in arbitrary units was plotted against protein concentrations. The graphs were created with SigmaPlot 8.0 (SPSS Inc.). RecF interactions with ssDNA (S1) are shown in panel A, with dsDNA (D1) in panel B, and with ds/ssDNA (J1) junction in panel C. Dotted lines represent the interaction of the RecF(S279R) mutant with DNA in the presence of ATP; short-dashed lines, a wild-type RecF in the presence of ADP; solid lines, a wild-type RecF in the presence of ATP; long-dashed lines, a wild-type RecF in the presence of ATP and RecR.

Journal:

Article Title: RecR-mediated Modulation of RecF Dimer Specificity for Single- and Double-stranded DNA * S⃞

doi: 10.1074/jbc.M806378200

Figure Lengend Snippet: RecF-DNA interaction measured by the fluorescence polarization assay. The labeled DNA substrates (20 nm) were titrated with RecF in the presence of 2 mm nucleotide. RecR was included where indicated at final concentration of 30 μm. Normalized fluorescence polarization signal in arbitrary units was plotted against protein concentrations. The graphs were created with SigmaPlot 8.0 (SPSS Inc.). RecF interactions with ssDNA (S1) are shown in panel A, with dsDNA (D1) in panel B, and with ds/ssDNA (J1) junction in panel C. Dotted lines represent the interaction of the RecF(S279R) mutant with DNA in the presence of ATP; short-dashed lines, a wild-type RecF in the presence of ADP; solid lines, a wild-type RecF in the presence of ATP; long-dashed lines, a wild-type RecF in the presence of ATP and RecR.

Article Snippet: The fluorescence polarization signal was plotted against the RecR concentration for each RecF concentration, then analyzed with SigmaPlot 8.0 (SPSS, Inc.) using a single-site ligand-interaction model and Equation 7 , (Eq.

Techniques: Fluorescence, Labeling, Concentration Assay, Mutagenesis